全文获取类型
收费全文 | 247篇 |
免费 | 6篇 |
国内免费 | 2篇 |
专业分类
林业 | 21篇 |
农学 | 5篇 |
基础科学 | 1篇 |
45篇 | |
综合类 | 7篇 |
农作物 | 5篇 |
水产渔业 | 75篇 |
畜牧兽医 | 64篇 |
园艺 | 4篇 |
植物保护 | 28篇 |
出版年
2022年 | 1篇 |
2021年 | 7篇 |
2020年 | 6篇 |
2018年 | 5篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 9篇 |
2014年 | 20篇 |
2013年 | 27篇 |
2012年 | 9篇 |
2011年 | 16篇 |
2010年 | 16篇 |
2009年 | 8篇 |
2008年 | 13篇 |
2007年 | 12篇 |
2006年 | 11篇 |
2005年 | 8篇 |
2004年 | 16篇 |
2003年 | 23篇 |
2002年 | 13篇 |
2001年 | 7篇 |
2000年 | 6篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1974年 | 1篇 |
1972年 | 2篇 |
排序方式: 共有255条查询结果,搜索用时 687 毫秒
31.
Takashige ISHII Kenji KAWASHIMA Haruo ORIBE Hiromi UEDA Toshiya HASUNUMA Kiyoshi AKIYAMA Hirofumi NAKAYAMA Mitsunori KURIHARA Fuminori TERADA Shiro KUSHIBIKI 《Animal Science Journal》2011,82(6):741-746
To decrease the age at first calving in Holsteins, the effects of average daily body weight gain (ADG) and crude protein (CP) level until first insemination on growth performance and milk production were examined. The MM group had a target ADG of 0.75 kg and received a diet with a CP level of 14%. The HM and HH groups had a target ADG of 1 kg; both these groups received a diet with CP levels 14% and 16%, respectively. The ADG in the HM and HH groups was 1.1 kg, whereas in the MM group it was 0.97 kg (P < 0.01). The HM and HH groups showed no differences in withers height at body weight 350 kg. The ages at first calving in MM, HM and HH groups were 23.1, 21.0 and 21.8 months, respectively. The HM and HH groups had lower milk yield at day 305 than the MM group (P < 0.01). These results suggest that growth performance until first insemination should be maintained at an ADG of 0.97 kg or less with a CP level of approximately 14%, to shorten time until first insemination and prevent the decrease of milk yield. 相似文献
32.
33.
Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate,suppresses FcepsilonRI expression in human basophilic KU812 cells 总被引:3,自引:0,他引:3
Fujimura Y Tachibana H Maeda-Yamamoto M Miyase T Sano M Yamada K 《Journal of agricultural and food chemistry》2002,50(20):5729-5734
We previously found that the O-methylated derivative of (-)-epigallocatechin-3-O-gallate (EGCg), (-)-epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG' '3Me), has potent antiallergic activity. The high-affinity IgE receptor, FcepsilonRI, is found at high levels on basophils and mast cells and plays a key role in a series of acute and chronic human allergic reactions. To understand the mechanism of action for the antiallergic EGCG' '3Me, the effect of EGCG' '3Me on the cell surface expression of FcepsilonRI in human basophilic KU812 cells was examined. Flow cytometric analysis showed that EGCG' '3Me was able to decrease the cell surface expression of FcepsilonRI. Moreover, immunoblot analysis revealed that total cellular expression of the FcepsilonRI alpha chain decreased upon treatment with EGCG' '3Me. FcepsilonRI is a tetrameric structure comprising one alpha chain, one beta chain, and two gamma chains. The level of mRNA production of each subunit in KU812 cells was investigated. EGCG' '3Me reduced FcepsilonRI alpha and gamma mRNA levels. The cross-linkage of FcepsilonRI causes the activation of basophils, which leads to the secretion of inflammatory mediators including histamine. EGCG' '3Me treatment inhibited the FcepsilonRI cross-linking-induced histamine release. These results suggested that EGCG' '3Me can negatively regulate basophil activation through the suppression of FcepsilonRI expression. 相似文献
34.
太行山小流域降雨—径流关系及其过程的研究 总被引:4,自引:0,他引:4
观测了水源区的太行山小流域径流及降雨过程 ,分析了径流与降雨的关系。结果表明 ,流域降雨季节性变化很大 ,主要集中于 6~ 9月 ,总量达 3 89.2 mm ,占 86.5 % ,11月到翌年 5月仅 60 .75 mm。在旱季 ,流域径流量逐渐下降 ,11月到翌年 5月底减少 0 .0 0 2 3 m3/ s,此期降雨次数和量较少 ,径流量持续下降 ,11月至翌年 3月由于植物叶片枯落 ,浅层土壤水经长时间蒸发后对深层裂隙水无明显影响 ,径流量较稳定 ,进入生长季后 ,流量近至枯竭。雨季降雨是流域径流的主要来源 ,当降雨量小于 5 0 mm时 ,降雨对径流的影响很小。连续暴雨使径流迅速形成洪峰 ,在平均雨强达到 2 8.91mm / h后 ,4 h径流量达最大值 0 .90 5 m3/ s,此期径流量与累积降雨量呈极显著的线性正相关。但洪峰仅持续 1~ 2 h,后期 5 7.3 m m的降雨量并未再次引起洪峰。 相似文献
35.
Hiroshi FUJISE Masaru MURAKAMI Bhuminand DEVKOTA Ishwori Prasad DHAKAL Kumiko TAKEDA Hirofumi HANADA Hideo FUJITANI Masao SASAKI Kosaku KOBAYASHI 《Animal Science Journal》2003,74(1):1-5
Lulu is a dwarf cattle population bred in the Mustang district of western and central Nepal. This area is located around the habitat boundary between Bos taurus and Bos indicus. The peculiarities of Lulu are their small size (weight range in the adult female: 68–153 kg) and rearing in high mountain areas at 2800 m to 4000 m in altitude. There were 5770 head of Lulu cattle in the Mustang district in 1998, 4333 females and 1437 males. The morphological appearance of Lulu is Bos taurus. However, one of the five Lulu studied in Kagbeni, Mustang had a Bos indicus mitochondrial DNA type based on the D‐loop sequence, while the other four were Bos taurus. It is suggested that there are hybrids of Lulu with Bos indicus maternal lineage in a mostly taurine‐breed genetic background. Steps must be taken to preserve the unique Lulu. 相似文献
36.
Nozaki H Wijayagunawardane MP Kodituwakku SP Yoshida T Nakamura T Arai I Urashima T Miyamoto A 《The Journal of reproduction and development》2005,51(2):229-234
N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was found in media cultured with bovine oviduct epithelial cells (BOEC) obtained from non-pregnant cows during the follicular phase. Combined treatment with specific hormones increased GnT I release from BOEC. Luteinizing hormone (LH; 10 ng/ml) alone slightly, but together with 17beta-estradiol (E2; 1 ng/ml), synergistically increased GnT I activity. Vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF) alpha, which have been shown to have their highest activities in the bovine oviduct during the periovulatory period, also increased in GnT I activity. This study provides the first evidence of an increase of GnT I release from BOEC in vitro, and shows that endocrine as well as local factors such as LH, VEGF and TNFalpha increase this activity. The results suggest that GnT I activity in the bovine oviduct may contribute to the induction of glycosylation and thereby contributing to the provision of the optimal microenvironment for fertilization and early development of the embryos. 相似文献
37.
Maeda H Shibuya H Suzuki K Kuwabara M Tsukise A Sato T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(11):1177-1180
A tumor sized in 2.0x2.0x2.5 cm developed in the cerebellum of a female Beagle was pathologically investigated. Histopathologically, the tumor grew by compression and partially by infiltration into the adjacent cerebellar parenchyma. There were a large number of necrotic lesions and proliferation of collagen fibers. The tumor cells had oval nucleus showing cellular atypia and a high mitotic index. The tumor cells were reacted with vimentin antibody on immunostain. Electron microscopic examination revealed the tumor cells interdigitated with cytoplasmic processus where the desmosomes developed on cell junction. This tumor was diagnosed as anaplastic meningioma, which is rarely observed in dogs. 相似文献
38.
Yano S Umeda D Maeda N Fujimura Y Yamada K Tachibana H 《Journal of agricultural and food chemistry》2006,54(14):5203-5207
Flavonoids ubiquitously exist in plants, vegetables, fruits, and teas. We evaluated the effect of dietary apigenin, one of the well-known flavonoids, on the immune system in C57BL/6N mice. Mice were fed experimental diets containing apigenin for 2 weeks. After the experimental period, there was no significant difference in body and organ weights between the control and the apigenin group. The total immunoglobulin (Ig) E levels in mice fed apigenin were significantly suppressed, whereas levels of IgG, IgM, and IgA were not affected. We also examined the effect of the apigenin diet on cytokine expression in mice sera using a cytokine array. The production of regulated upon activation normal T cell expressed and secreted (RANTES) and soluble tumor necrosis factor receptor I (sTNFRI) in mice sera was down-regulated by the apigenin diet. These results suggest that a diet containing apigenin can reduce serum IgE and inflammatory cytokines such as RANTES and sTNFRI in mice. 相似文献
39.
Yoshie MANABE Makoto TOCHIGI Akiyoshi MORIWAKI Sakae TAKEUCHI Sumio TAKAHASHI 《The Journal of reproduction and development》2013,59(4):398-404
Reproductive functions decline with the onset of diabetes in female mice. Diabetic mice
have smaller uteri with an underdeveloped endometrium, suggesting diminished
estrogen-induced growth. We aimed to clarify the changes in the estrous cycle and in
insulin-like growth factor 1 (IGF1) expression in the uteri of streptozotocin
(STZ)-treated diabetic mice, because IGF1 is one of the main growth factors involved in
estrogen-induced uterine growth. ICR female mice were intraperitoneally administered STZ
(10 mg/100 g BW), and blood glucose levels were determined. Mice with blood glucose levels
> 200 mg/dl were classified as diabetic mice. The onset of diabetes was associated with
acyclic estrous cycles. Diabetes was also induced with STZ in ovariectomized mice. Uterine
Igf1 mRNA levels were reduced in ovariectomized STZ-treated diabetic
mice. Estrogen is known to stimulate Igf1 mRNA expression in the uterus,
but estrogen action was abolished in the uteri of STZ-treated diabetic mice. mRNA
expressions of estrogen receptor α (ERα) and steroid hormone receptor coactivators
(SRC-1/Ncoa1, SRC-2/Ncoa2,
SRC-3/Ncoa3 and CBP/p300/Crebbp) were reduced in the
uteri of ovariectomized STZ-treated diabetic mice. The present study demonstrates that
diabetes induces a decline in female reproductive functions in mice. Igf1
expression in ovariectomized diabetic female mice was decreased, and decreased
responsiveness to estrogen in the uteri of diabetic mice is probably associated with a
reduction in ERα and steroid receptor coactivator mRNA expression. 相似文献
40.
Molecular characterization of an H1N2 swine influenza virus isolated in Miyazaki, Japan, in 2006 总被引:1,自引:0,他引:1
Saito T Suzuki H Maeda K Inai K Takemae N Uchida Y Tsunemitsu H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):423-427
Swine influenza virus (SIV) was isolated from a farm in Miyazaki Prefecture in Japan in July 2006. An isolate was genetically subtyped as H1N2 and was designated A/swine/Miyazaki/1/2006 (H1N2). The nucleotide sequences of all eight viral RNA segments were determined, and then phylogenetic analysis was performed using the neighbor-joining method. All segments were shown to be closely related to those of Japanese SIV H1N2 isolates, which have been circulating since the 1980s. The results indicate the persistence of the SIV H1N2 subtype in the Japanese pig population for more than two decades and emphasize the importance of continuous surveillance for SIV. 相似文献